SensiFAST SYBR No-ROX Kit
CAT# PRODUCT SIZE STORAGE Һڰ ֹ
BIO-98005SensiFAST SYBR No-Rox Kit500 RXNSat -20C-α
BIO-98020SensiFAST SYBR No-Rox Kit2000 RXNSat -20C-α
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"In the course of optimizing real-time PCR for analysis of various animal tissues, I tested multiple real-time master mixes of different brands. The SensiFAST SYBR No-ROX Kit produced the most valid amplification result by improving efficiency and reproducibility. With this product, Bioline offers an optimal cost-performance ratio."
 
 
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Real-time PCR ϰ ΰ м ϳ ǿ ǰ ֽϴ. Ư nucleic acid ϰ ϴµ ־ ȿ mRNA miRNA , biomarker ߰߰ validation, Microarraying, ̻ , 赵 , gene dosage , 翡 ſ پ о Ͽϴ. Real-time PCR ü ð high throughput assay 䱸 Խϴ. ̷ 䱸 ȿ(Ȯ ) PCR ̴ ̷ϴ. ο SensiFAST Kits ̷ ޾Ƶ̱ ߵǾ antibody hot-start enzyme ſ ռ ӵ Ͽ fast cycling ϰ Ͽϴ. SensiFAST standard qPCR , ŷڼ, ϸ鼭 cycling time մϴ. SensiFAST real-time ϸ Ư fast cycler մϴ.
 
 

Features and Benefits

• Ȯ - hot-start primer-dimer
• ΰ - target
• - ְ Ư̼ ΰ Ư
• ȣȯ - fast cycling PCR real-time PCR ȣȯ
• Protocol - fast qPCR standard qPCR protocol
 

Instrument Compatibility

Roche LightCycler 480 platform, Bio-Rad Opticon, Opticon2, Chromo4, MiniOpticon, CFX96, CFX384, Cepheid SmartCycler, Qiagen Rotor-Gene 3000, 6000, Eppendorf Mastercyclerep realplex, Illumina Eco, Takara Thermal Cycler Dice (TP800) and Techne Quantica (See product selection table)

Description

SensiFAST SYBR Kits real-time PCR ߵǾ Ϲ ǰ ִ real-time 迡 Ǿϴ. ֽ buffer enhancer׸ antibody hot-start DNA Polymerase SensiFAST SYBR kit ְ ΰ real-time PCR ϵ մϴ. ϱ ϵ SensiFAST SYBR Kit 2X matermix type Ǹ real-time PCR ʿ SYBR Green I Dye, dNTPs, stabilisers enhancers ԵǾ ֽϴ. kit DNA template ϰ RNA template Bioline Tetro cDNA Synthesis Kit(BIO-65042) reverse
transcription մϴ. 18~19page compatibility table ִ 迡 SYBR Green-based real-time PCR 뿡 ȭǾϴ. ŰƮ ROX ʴ ͵ ȣȯ մϴ. Qiagen(Corbett), Rotor-Gene 6000, Bio-Rad CFX 96, Roche LightCylcer 480) SensiFAST SYBR Hi-ROX Lo-ROX kit û premixed ROX ԵǾ ֽϴ. SensiFAST SYBR & Fluorescein Kit premixed Fluorescein ԵǾ ֽϴ.
 

Volume

BIO-98002: 200 x 20µl Reactions: 2 x 1ml
BIO-98005: 500 x 20µl Reactions: 5 x 1ml
BIO-98020: 2000 x 20µl Reactions: 20 x 1ml
 

Components

2x Reaction mix consisting of: Heat-activated DNA polymerase Ultra-pure dNTPs MgCl2 SYBR Green I

 

Kit Size

The kit size is based on a 20µl final reaction volume.

 

Storage Conditions

12 months at -20C. Avoid exposure of the SYBR Green I to light.

 

Notes

SensiFAST is a trademark of Bioline Reagents Ltd.

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SensiFAST SYBR Hi-ROX Kit
SensiFAST SYBR Lo-ROX Kit
SensiFAST SYBR & Fluorescein Kit
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