MyTaq Red DNA Polymerase
CAT# PRODUCT SIZE STORAGE Һڰ ֹ
BIO-21108MyTaq Red DNA Polymerase500 UNITSat -20C-α
BIO-21109MyTaq Red DNA Polymerase2500 UNITSat -20C-α
BIO-21110MyTaq Red DNA Polymerase5000 UNITSat -20C-α
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"We found MyTaq to be easy to use, reasonably priced and performs well under various conditions."

 
- Dublin City University, Ireland
 

Features


New generation polymerase with superior performance Increased sensitivity and speed Novel buffer system, including dNTPs and MgCl2 Robust and high yield across a wide range of templates Includes red dye for direct gel loading

 

Applications


High-throughput PCR Specific amplification of complex templates Robust amplification of GC-rich sequences TA cloning

 

Description


MyTaq Red DNA Polymerase is a high-performance PCR product that exhibits more robust amplification than other commonly used polymerases (Fig. 1). MyTaq Red DNA Polymerase delivers very high yield over a wide range of PCR templates, making MyTaq Red DNA Polymerase the ideal choice for most routine assays.

This new enzyme preparation from Bioline is supplied with a 5x MyTaq Red Reaction Buffer system, a proprietary formulation that saves time (Fig. 2) and delivers superior results. MyTaq buffer contains dNTPs, MgCl2 and enhancers at optimal concentrations that eliminate the need for further optimization.

The specially designed MyTaq Red formulation does not interfere with PCR reactions and enables users to load samples directly onto a gel after the PCR without the need to add loading buffer. The red dye migrates like a 350bp fragment on a 2% agarose TAE gel (or 600bp on a 1% agarose).

MyTaq Red only requires the addition of template, primers and water, thus reducing the risk of pipetting errors and contamination, as well as shortening the set-up time.

 

Components

MyTaq DNA Polymerase 5x MyTaq Reaction Buffer

Unit Definition

One unit is defined as the amount of enzyme that incorporates 10nmoles of dNTPs into acid insoluble form in 30 minutes at 72C.

 

Concentration


5u/l

 

Storage Conditions


MyTaq Red DNA Polymerase can be stored for up to 6 months at -20C, or up to 2 weeks at +4C. Repeated freeze/thaw cycles should be avoided.

 

Notes


MyTaq is a trademark of Bioline.
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