Features and Benefits• ºü¸§ - ºü¸¥ reverse transcription real-time PCR¿¡ ÃÖÀûÈ • Á¤È®ÇÑ Á¤·® - low copy RNA¿¡ ÃÖÀû • ¹Î°¨µµ - µ¶Æ¯ÇÑ buffer Á¶¼ºÀ¸·Î ºü¸¥ Ct °ª • ȣȯ¼º - ¸ðµç fast cycling instruments¿Í ȣȯ • Protocol - fast PCR °ú standard PCR protocol ¸ðµÎ °¡´É Instrument Compatibility7500, 7500 FAST, Stratagene (Agilent) Mx4000¢â, Mx3000P¢â, Mx3005P¢â, as well as other instruments that do not require the use of a passive reference. DescriptionSensiFAST Probe One-Step Kits´Â ºü¸¥ RT-qPCRÀ» À§ÇØ °³¹ßµÇ¾úÀ¸¸ç ÀϹÝÀûÀ¸·Î »ç¿ëµÇ°í ÀÖ´Â real-time ±â°è¿¡¼ °ËÁõÀÌ µÇ¾ú½À´Ï´Ù. ÀÌ Å°Æ®´Â PrimeTime, TaqMan, Scorpions, Molecular Beacon probe¸¦ Æ÷ÇÔÇÑ probe-detection ±â¼ú»ç¿ë¿¡ ¶Ù¾î³ ¹Î°¨µµ¿Í ƯÀ̼ºÀ» °¡Áöµµ·Ï Á¶¼ºµÇ¾ú½À´Ï´Ù. SensiFAST SYBR One-Step Kits´Â single tube¿¡¼ ³ôÀº Àç»ý¼ºÀÇ first strand cDNA¿Í ÈÄ¼Ó real-time PCRÀÌ °¡´ÉÇϵµ·Ï Á¶¼ºµÇ¾ú°í ÃÖ½ÅÀÇ buffer Á¶¼º°ú enhancer ±×¸®°í antibody ±â¹Ý hot-start DNA PolymeraseÀÇ Á¶ÇÕÀº SensiFAST Probe kit°¡ ºü¸£°í Àç»ý°¡´ÉÇϸç ÃÖ°í·Î ¹Î°¨ÇÑ one step RT-qPCRÀÌ °¡´ÉÇϵµ·Ï ÇÕ´Ï´Ù. ¶ÇÇÑ SensiFAST Probe One-StepÀº multiplexing¿¡µµ ´ëÀûÇÒ ¼ö ¾ø´Â È¿À²¼ºÀ» º¸ÀÔ´Ï´Ù. SensiFAST Probe One-step kit´Â 2X SensiFAST Probe One-Step mix¿Í º°µµÀÇ reverse transcriptase, RiboSafe Inhibitor·Î ±¸¼ºµÇ¾î ÀÖÀ¸¸ç SensiFAST Probe Hi-ROX¿Í Lo-ROX One-Step Kit´Â ¼±Åûç¿ëÀ» À§ÇØ premixed ROX°¡ Æ÷ÇԵǾî ÀÖ½À´Ï´Ù. VolumeBIO-78001: 100 x 20µl Reactions: 1 x 1ml BIO-78005: 500 x 20µl Reactions: 5 x 1ml Components2x Reaction mix consisting of: Heat-activated DNA polymerase
Ultra-pure dNTPs
MgCl2
Plus separate vials of: Reverse Transcriptase
RNase inhibitor
DEPC-treated water
Kit SizeThe kit size is based on a 20µl final reaction volume. Storage Conditions12 months at -20¡ÆC. NotesSensiFAST is a trademark of Bioline Reagents Ltd. protocol [pi_50203_sensifast_probe_lo_rox_one_step_kit_v1.pdf] (442,954kb) |
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