TransIT-X2 Dynamic Delivery System
CAT# PRODUCT SIZE STORAGE Һڰ ֹ
MIR6003[Mirus]TransIT-X2 Dynamic Delivery System0.3 ml-α
MIR6004[Mirus]TransIT-X2 Dynamic Delivery System0.75 ml-α
MIR6000[Mirus]TransIT-X2 Dynamic Delivery System1.5 ml-α
MIR6005[Mirus]TransIT-X2 Dynamic Delivery System5 x 1.5 ml-α
MIR6006[Mirus]TransIT-X2 Dynamic Delivery System10 x 1.5 ml-α
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NEW! TransIT-X2 Dynamic Delivery System

A novel, polymeric system for efficient delivery of plasmid DNA and/or siRNA/miRNA

 
TransIT-X2 Dynamic Delivery System non-liposomal, polymeric system ̿Ͽ primary cell پ cell type پ ȿ Դϴ.
TransIT-X2 Dynamic Delivery System DNA siRNA/miRNA transfection ̿ , DNA siRNA/miRNA transfection մϴ.
TransIT-X2 transfectionĿ ȯ ʿ , serum Ե transfection մϴ.
TransIT-X2 Dynamic Delivery System cell type transfection Lipofectamine2000 ǰ ֽϴ.
 
  • ȿ transfection .
  • ȯ plasmid DNA siRNA co-transfection .
  • â non-liposomal, polymeric technology.
Figure 1:Visualization of High GFP Expression using TransIT-X2 Dynamic Delivery System
Figure 1. Visualization of High GFP Expression using TransIT-X2 Dynamic Delivery System. TransIT-X2 Dynamic Delivery System was used to transfect plasmid DNA encoding EGFP into A549, CHO-K1, HepG2, LNCaP, MDCK, PC12, primary human mammary epithelial cells (HMEC) and normal human dermal fibroblasts (NHDF). Transfections were performed in 35 mm MatTek dishes using 4-8 µl of TransIT-X2 to deliver 2 µg of DNA. Images (32X) were captured at 48 hours post-transfection using a Zeiss Axiovert S100 inverted fluorescence microscope.

* indicates primary cell types
Figure 2. High GFP Transfection Efficiency in Multiple Cell Lines and Primary Cells using TransIT-X2 Dynamic Delivery System. TransIT-X2 Dynamic Delivery System was used to transfect plasmid DNA encoding EGFP into A549, CHO-K1, Hep G2, MDCK, LNCaP, PC-12, primary human mammary epithelial cells (HMEC) and normal human dermal fibroblasts (NHDF). Transfections were performed in 96-well plates using 0.2-0.4 µl of TransIT-X2 to deliver 0.1 µg of DNA (2:1, 3:1 or 4:1 reagent: DNA ratio). Triplicate wells were assayed 48 hours post-transfection using guava easyCyte 5HT Flow Cytometer.

*indicates primary cell types
 
Figure 3. Functional Co-delivery of Plasmid DNA and siRNA using the TransIT-X2 Dynamic Delivery System. TransIT-X2 Dynamic Delivery System was used to transfect plasmid Cy5 labeled DNA encoding nuclear YFP and Cy3 labeled siRNA into HeLa cells. Transfection was performed in 6-well plates with Poly-L-Lysine (PLL) coated coverslips using 4 µl of TransIT-X2 to deliver 2 µg of DNA and 25 nM siRNA (2:1 reagent:DNA ratio). Actin cytoskeleton was stained using Alexa Fluor 350 Phalloidin. Images (63X) were captured at 24 hours post-transfection using a Nikon A1R confocal microscope. Image key: yellow (nuclear YFP), blue (Cy5 labeled DNA), red (Cy3 labeled siRNA), green (actin cytoskeleton).
 
Figure 4. TransIT-X2 Dynamic Delivery System Achieves Higher Knockdown than Lipofectamine 2000. TransIT-X2 Dynamic Delivery System and Lipofectamine 2000 Transfection Reagent were used to transfect siRNA targeting endogenous proteins – GAPDH and AHA1 or to deliver a non-targeting siRNA control in normal human dermal fibroblasts (NHDF). Cells were transfected in a 6-well plate using 4 µl of TransIT-X2 or 6 µl of Lipofectamine 2000 and 25 nM siRNA according to each manufacturer's protocol. The amount of GAPDH or AHA1 mRNA was measured relative to 18s rRNA levels using qRT-PCR and then normalized to the mRNA levels of the non-targeting control, 48 hours post-transfection. Error bars represent the standard deviation of triplicate wells.
 
 
Figure 5. Effective miRNA Delivery using TransIT-X2 Dynamic Delivery System Yields Decreased Levels of PTK9 mRNA. TransIT-X2 Dynamic Delivery System and Lipofectamine 2000 Transfection Reagent were used to transfect Pre-miR hsa-miR-1 miRNA Precursor or mirVana miRNA mimic, miR-1, both known to decrease PTK9 mRNA levels. A Pre-miR negative control was also transfected to assess baseline mRNA levels. T47D cells were transfected in a 12-well plate using 3 µl of TransIT-X2 or Lipofectamine 2000 and 50 nM miRNA according to each manufacturer's protocol. The amount of PTK9 mRNA was measured relative to 18s rRNA levels using qRT-PCR and then normalized to the mRNA levels of the negative control, 48 hours post-transfection.  Error bars represent the standard deviation of triplicate wells.
Storage Conditions: Store at -20C
Product Guarantee: 6 months
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X2 ı - ڷп OOڻ (A549 cell)
cell viability - lipofectamine 2000 X2
siRNA delivery test - RNAimax x2 . (A549)
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