SensiMix II Probe Kit
CAT# PRODUCT SIZE STORAGE ¼ÒºñÀÚ°¡ ÁÖ¹®
BIO-83005SensiMix II Probe mastermix 2x500 RXNSat -20C¡Æ-·Î±×ÀÎ
BIO-83020SensiMix II Probe mastermix 2x2000 RXNSat -20C¡Æ-·Î±×ÀÎ
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"I have reviewed numerous real-time PCR master mixes and I would like to share with you that I am making a resounding endorsement of SensiMix Probe II!"

 
- Yale University
School of Forestry and Environmental Studies
 

Features and Benefits

 
³ôÀº PCR È¿À² : sequence¿¡ ƯÁ¤È­µÈ Probes
°í°¨µµ : ÁÙ¾îµç background¿Í ³ôÀº »ý»ê¼º
°£´ÜÇÏ¸ç ³ôÀº »ý»ê¼º : multiplexing°ú gene expression È®ÀÎ ½Ã ÃßõÇÕ´Ï´Ù
Flexible : ¸ðµç real-time PCR Àåºñ¿¡ »ç¿ë °¡´ÉÇÕ´Ï´Ù.

Instrument Compatibility


Hi-rox :

ABI 7000, 7300, 7700, 7900, 7900HT, 7900HT FAST, StepOne¢â, StepOne¢â Plus (see product selection table) each of these instruments having the capacity to analyze the real-time PCR data with the passive reference signal either on or off, as well as several instruments that do not require the use of ROX.

Lo-rox :
ABI 7500, 7500 FAST, ViiA7¢â, Stratagene (Agilent) Mx4000¢â, Mx3000P¢â, Mx3005P¢â (see product selection table), as well as other instruments that do not require the use of a passive reference.
No-rox :
Roche LightCycler¢ç 480 platform, LightCycler¢ç Nano, Bio-Rad Opticon¢â, Opticon¢â2, Chromo4¢â, MiniOpticon¢â, CFX96¢â, CFX384¢â, Cepheid SmartCycler¢ç, Qiagen Rotor-Gene¢â 3000, Rotor-Gene¢â 6000, Rotor-Gene¢â Q, Eppendorf Mastercycler¢çep realplex, Illumina¢ç Eco¢â, Takara Thermal Cycler Dice¢ç (TP800), Analytik Jena qTOWER and Techne Quantica¢ç, PrimeQ.

Description


SensiMix¢â II Probe Kit ´Â ¸ðµç real-time Àåºñ¿¡¼­ ³ôÀº °¨µµ¿Í ´õºÒ¾î ƯÀ̼ºÀ» °®´Â °í¼º´ÉÀ» ÀÚ¶ûÇÏ´Â ½Ã¾àÀÔ´Ï´Ù. TaqMan¢ç, Scorpion¢ç, Assay On Demand¢ç, allelic discrimination °ú molecular beacon probesµîÀ» Æ÷ÇÔÇÏ´Â probe-detection ±â¼ú¿¡ »ç¿ëµÇ°Ô²û ¸¸µé¾îÁø ÀÌ ½Ã¾àÀº ƯÈ÷ gene expression analysis¿Í multiplexingÀ» ÇÒ ¶§ ¼º°øÀûÀÎ ½ÇÇè °á°ú¸¦ °¡Á®´Ù ÁÝ´Ï´Ù. SensiMix¢â II Probe Kit´Â hot-start DNA polymerase¸¦ Æ÷ÇÔÇϴµ¥ ÀÌ enzymeÀ¸·Î ÇÏ¿©±Ý ³ôÀº PCR °¨µµ¿Í Á¤È®µµ¸¦ ±¸»çÇÏ°Ô ÇØ ÁÝ´Ï´Ù. Hot start DNA polymerase°¡ °®´Â Ư¼º¶§¹®¿¡ ½ÇÇè ¼¼ÆÃÇÏ´Â µ¿¾È¿¡´Â È°¼ºÀ» °®Áö ¾Ê½À´Ï´Ù. µû¶ó¼­ ÀÌ·± Ư¼ºÀ¸·Î ÀÎÇØ non specificÇÑ ÁõÆøÀ̳ª primer-dimer »ý¼ºÀ» ¸·À» ¼ö ÀÖ½À´Ï´Ù. Pre-heatingÀÌ ³¡³­ ÈÄ¿¡¾ß SensiMix °¡ ¿Ïº®ÇÏ°Ô È°¼ºÀ» ¶ç°Ô µÇ°í ÀÌ enzyme¿¡ ¸Â°Ô Ư¼ºÈ­µÈ buffer Á¶¼º°ú ´õºÒ¾î ½Å·Ú ÇÒ ¼ö ÀÖ´Â °í Ä÷¸®Æ¼ÀÇ µ¥ÀÌÅ͸¦ ¾òÀ¸½Ç ¼ö ÀÖ½À´Ï´Ù.
½ÇÇèÀÚÀÇ Æí¸®¼ºÀ» À§ÇØ SensiMix II Probesms 2x mastermix ŸÀÔÀ¸·Î Á¦°øµÇ´Âµ¥ dNTPs¿Í stabillizers¸¦ Æ÷ÇÔÇÑ real-time PCR¿¡ ÇÊ¿äÇÑ ¸ðµç ÇÊ¿ä½Ã¾àÀÌ Æ÷ÇԵǾî ÀÖ½À´Ï´Ù.

Volume


 500 x 50µl Reactions: 10 x 1.25ml
2000 x 50µl Reactions: 40 x 1.25ml

Kit Size


The kit size is based on a 50µl final reaction volume.

Notes


SensiMix is a trademark of Bioline Reagents Ltd.

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SensiMix II Probe Kit
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