Click ChemistryClick chemistry is a two-step process that uses quantitative chemical reactions of alkyne and azide moieties to create covalent carbon-heteroatom bonds between biochemical species.1 The reaction uses copper(I) as a catalyst and forms a 1,2,3-triazole between an azide and terminal alkyne.2 The technology is reliable and stable which makes it an ideal oligonucleotide labeling method.
The benefits of click chemistry include1:
Modifications Available at IDT:
To order an alkyne or azide modified oligo:
Specify the nucleic-acid sequence along with the appropriate alkyne or azide modifications included in the sequence.
To order an oligo modified by a 1,2,3-triazole linkage of Biotin, 6-FAM, or 5-TAMRA :
Select the desired azide modification at the desired location within the nucleic-acid sequence. IDT will synthesize the oligo, perform the click chemistry reaction, and purify the product. References
Click-Chemistry enabled, modified oligos are manufactured and sold under license from Baseclick GmbH, using Base-click's proprietary Click Chemistry. All such modified products are sold by IDT for the end-user's internal research purposes only. See www.baseclick.org for further details. |
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