miRCat¢ç Small RNA Cloning KitmiRCat¢ç small RNA cloning kit´Â pre-activated, adenylated RNA linkering ¹æ¹ý¿¡ ±Ù°ÅÇÑ °ÍÀ¸·Î ¾î¶² Á¾¿¡¼ À¯·¡µÈ ¾î¶² RNA source·ÎºÎÅ͵µ Ŭ·Î´×ÀÌ °¡´ÉÇÏ°Ô ÇÕ´Ï´Ù. miRCat-33¢ç kit´Â 5' ligation-independent small RNA cloningÀ» ¼öÇàÇϱâ À§ÇÑ ¸ñÀûÀ¸·Î miRCat¢ç kit ¸¦ º¯ÇüÇÑ °ÍÀÔ´Ï´Ù.
miRCat¢ç Small RNA Cloning Kit
miRCat¢ç kit¿¡´Â 10¹øÀÇ Å¬·Î´× ½ÇÇèÀ» ÇÒ ¼ö ÀÖ´Â ¾çÀÌ µé¾î ÀÖ½À´Ï´Ù.
miRCat¢ç small RNA cloning is based upon the pre-activated, adenylated RNA linking method that has been used successfully in many labs since its development in 2001 [1]. This method permits cloning from any RNA source in any species. The miRCat-33¢ç Conversion Kit allows the miRCat Kit to be used for 5¡Ç ligation-independent small RNA cloning using the method of Pak and Fire [2].
Material sufficient for ten cloning experiments is provided in the miRCat¢â kit.
Kit Contents:
Figure 1. The Process of Cloning miRNAs and Other Small RNAs from any Total RNA Source Using the miRCat¢ç Kit. The Ban I restriction enzyme sites in the cloning linkers are shown by cross hatching. The page numbers indicate the pages in the full length manual where each step of the protocol begins. The Ban I restriction enzyme sites in the cloning linkers are shown by cross hatching. The page numbers indicate the pages in the full length manual where each step of the protocol begins.References
miRCat-33¢ç Conversion KitEach oligo pack contains:
miRCat-33¢ç is a conversion of the miRCat¢ç kit for the purpose of carrying out 5¡Ç ligation-independent small RNA cloning using the method of Pak and Fire [1]. Primer is included at no charge. References
454 miRCat¢ç and miRCat-33¢ç Adapter Primers454 Adaptor Primers are designed to convert the small RNA libraries generated by miRCat ligations (Set I) or by miRCat-33 ligations (Set II) into 454-compatible PCR libraries for deep sequencing [1]. References
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