CAT#	PRODUCT	SIZE	STORAGE	소비자가	주문
30081-3	T4 DNA Polymerase	1500 U		-	로그인

T4 DNA Polymerase

Recombinant T4 DNA Polymerase
Many applications including creating blunt-ends and in vitro mutagenesis

T4 DNA Polymerase is a DNA-dependent DNA polymerase which catalyzes the 5’→3’ synthesis of DNA and requires template and primer. Additionally, the enzyme has a strong 3’→5’ exonuclease activity.

Source:
Purified from a strain of E. coli that carries a T4 DNA polymerase expression plasmid.

Applications:

Fill in of 5’ overhanging ends to create blunt-ends (1).
Exonuclease removal of 3’ overhanging to create blunt-ends (1).
in vitro mutagenesis (2)

Heat inactivation: 70°C for 15 minutes.

Storage:
Enzyme and buffer should be stored at -20°C. Enzyme is stable for 12 months if handled properly.

Additional Information:

T4 DNA Polymerase is active in many buffers and can often be used directly in a restriction digest reaction.
T4 DNA Polymerase exhibits no strand displacement activity.

References:

Tabor, S. and Struhl, K. (1989) In DNA-Dependent DNA Polymerases. F. M. Ausebel, R. Brent, R. E. Kingston, D. D. Moore, J. G. Seidman, J. A. Smith and K. Struhl (Eds.), Current Protocols in Molecular Biology, pp. 3.5.10-3.5.12.
Kunkel, T.A., Roberts, J.D. and Zakour, R.A. (1987) R. Wu and L. Grossman (Eds.), Methods Enzymol., 154, pp. 367-382. San Diego: Academic Press.

MA071-T4-DNA-Polymerase.pdf (103,793kb)

MSDS.pdf (118,131kb)