Custom Dicer-Substrate siRNAs (DsiRNAs)RNAi´Â target À¯ÀüÀÚÀÇ ¹ßÇöÀ» ƯÀÌÀûÀ¸·Î ¾ïÁ¦Çϱâ À§ÇØ short double-stranded (ds) RNAs¸¦ »ç¿ëÇÕ´Ï´Ù. ¼¼Æ÷ ³»¿¡¼´Â long dsRNA °¡
Dicer¿¡ ÀÇÇؼ 2-base 3'-overhangs ¸¦ °¡Áø short 21-23 base duplexes·Î À߸®°Ô µË´Ï´Ù. "small interfering RNAs" (siRNAs) ¶ó ºÒ¸®´Â ÀÌ·¯ÇÑ RNA´Â RNA Induced Silencing Complex (RISC)·Î µé¾î°¡°Ô µÇ°í »óº¸ÀûÀÎ mRNA¸¦ degradationÇϴµ¥ sequence-specific guide ¿ªÇÒÀ»ÇÏ°Ô µË´Ï´Ù. ¿ª»çÀûÀ¸·Î small interfering RNAs (siRNAs) ´Â 21-mers·Î ÇÕ¼ºÀÌ µÇ¾î in vivo DicerÀÇ »ê¹°À» Á÷Á¢ÀûÀ¸·Î Èä³» ³»µµ·Ï ÇÏ¿´½À´Ï´Ù. µû¶ó¼ ÀüÅëÀûÀÎ 21-mer siRNAs´Â Dicer cleavage°úÁ¤ÀÌ ¾øÀÌ Áö³ªÄ¡°Ô µË´Ï´Ù. ±×·¯³ª ÃÖ±Ù¿¡ Dicer´Â siRNA duplexes°¡ RISC·Î µé¾î°¡´Âµ¥ °ü°èÇÑ´Ù´Â °ÍÀ» ¾Ë·ÁÁö°í Àֱ⠶§¹®¿¡ Dicer¿ÍÀÇ interaction¿¡ ÇʼöÀûÀÎ ½ºÅÜÀ¸·Î º¸ÀÔ´Ï´Ù.
IDT»ç¿Í Beckman Research Institute of the City of Hope National Medical CenterÀÇ ±èµ¿È£ ¹Ú»ç¿Í John Rossi¹Ú»çÀÇ °øµ¿¿¬±¸·Î °³¹ßµÈ
Dicer-substrate RNAs´Â ÇÕ¼ºµÈ 27-mer duplex RNAs·Î¼ Dicer¿¡ ÀÇÇØ 21-mer siRNAs·Î ¸¸µé¾îÁö°Ô µË´Ï´Ù. ÀÌ·¸°Ô »õ·Ó°Ô µðÀÚÀÎ µÈ RNAiduplex´Â °°Àº »çÀÌÆ®¿¡ ´ëÇÏ¿© ±âÁ¸ÀÇ 21mer siRNAº¸´Ù 10¹è±îÁö Áõ°¡µÈ È¿À²À» ³ªÅ¸³À´Ï´Ù.
27 mer·ÎºÎÅÍ ÇÑ °³ ÀÌ»óÀÇ 21-mer product°¡ ¸¸µé¾î Áú ¼ö ÀÖÁö¸¸ ¸ðµÎ°¡ È¿À²À» Áõ°¡½ÃÅ°Áö´Â ¾Ê½À´Ï´Ù. IDT´Â È¿À²À» ±Ø´ëÈÇÏ´Â dicing µðÀÚÀÎ ÅøÀ» °³¹ßÇßÀ¸¸ç À¥ »ó¿¡¼ ¹«·á·Î ÀÌ¿ëÇÏ½Ç ¼ö ÀÖ½À´Ï´Ù. ÀÌ·¯ÇÑ ¼ºñ½º´Â ¿ÀÁ÷ IDT¿¡¼¸¸ °¡´ÉÇÕ´Ï´Ù
±æÀÌÁ¦ÇÑ: Dicer Substrate RNAi oligos´Â strand ´ç 24-30 RNA bases¿Í 3 DNA bases±îÁö °¡´ÉÇÕ´Ï´Ù.
DsiRNA º¸Àå·®
Predesigned DsiRNAs - DsiRNA RefSeq LibraryRefSeq Genbank collection(http://www.ncbi.nlm.nih.gov/RefSeq.) ¿¡¼ °¢°¢ÀÇ human, mouse, rat, cow, dog, chicken, and chimp
transcriptomesÀÇ ¾à 25,000°³ À¯ÀüÀÚÀÇ 3,000,000°³ ÀÌ»óÀÇ pre-designed DsiRNA¸¦ IDT¿¡¼ Á¦°øÇÕ´Ï´Ù. IDT¿¡¼´Â 3,000,000°³ ÀÌ»óÀÇ ¸ð
µç pre-designed DsiRNAÀÇ sequence¸¦ ¸ðµÎ Á¦°øÇØ ÁÖ°í ÀÖ½À´Ï´Ù. ÀÌ pre-designÀº IDT¸¸ÀÇ Dicer-substrate specificÇÑ ±ÔÄ¢À» ÀÌ¿ëÇÑ ¾Ë°í¸®ÁòÀ¸·Î ¸¸µé¾î Áý´Ï´Ù. °¢ sequencesµéÀº cross-hybridization°ú off-target effects(Smith-Waterman analysis)¸¦ ÃÖ¼ÒÈ ÇÏ¿´°í ÀÌ¹Ì ¾Ë·ÁÁø SNP³ª alternative splice exonºÎÀ§´Â Á¦°ÅµÇ°í µðÀÚÀÎ µË´Ï´Ù. ¸¶Áö¸·À¸·Î local mRNAÀÇ 2Â÷ ±¸Á¶µµ ÇÇÇϵµ·Ï ÇÕ´Ï´Ù.
IDT´Â µÎ °¡Áö Á¾·ùÀÇ Predesigned DsiRNA duplexes¸¦ Á¦°øÇÕ´Ï´Ù.
••Splice common: targets all known variants of a gene in RefSeq; duplexes lie only within common extons. This constitutes the bulk of the DsiRNA collection and is the correct choice for most users. 8-10 duplexes are available for each gene target.
••Splice specific: targets only exons present in specific splice forms. In some cases, the unique exon may be very small or comprise sequence unfavorable for selection of RNAi duplexes (please note that knock-down guarantees do not apply for these sites). This collection is intended for those researchers who have a specialized need to selectively target specific splice variants or isoforms.
µðÀÚÀÎÀº ¸µÅ©µÈ µðÀÚÀÎ Åø¿¡¼ Á÷Á¢ È®ÀÎ ÇÒ ¼ö ÀÖ½À´Ï´Ù.http://sg.idtdna.com/Scitools/Applications/Predesign/ |
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