Tet expression systemFeatures and benefits of the pASK-IBA vectors:
Principle and properties
T7 expression systemFeatures and benefits
Principle and propertiesThe Tet promoter is of medium strength which leads to high level expression of certain proteins depending e.g. on their folding rate and stability - characteristics which can hardly be predicted. Some proteins, however, can only be expressed at high level if transcribed by a stronger promoter. In such cases we recommend the use of the T7 promoter.
The T7 expression system is encoded on the pPR-IBA vectors. The system uses the T7 promoter and T7 RNA polymerase for high-level transcription of the gene of interest. Expression of the target genes is induced by providing a source of T7 RNA polymerase in the host cell. This is accomplished by using E. coli BL21 which contains a chromosomal copy of the T7 RNA polymerase gene. The latter is under control of the lacUV5 promoter which can be induced by IPTG. ReferencesTet promoter
Tet promoter:
pASK-Vectors
pASK-Vectors:
pPR-IBA vectors
pPR-IBA vectors:
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