Strep-tag¢ç antibodiesThe following monoclonal antibodies against Strep-tag¢ç are available:
Further products:
StrepMAB-ClassicStrepMAB-Classic is recommended for the following applications:
StrepMAB-Classic, horseradish peroxidase (HRP) conjugateThe StrepMAB-Classic, HRP conjugate1 is recommended for detection of Strep-tag¢ç II proteins in Western blots without the need for secondary antibodies. 1The conjugate is useful for both chemiluminescence and chromogenic detection.
StrepMAB-ImmoHigh-affinity Strep-tag¢ç II specific monoclonal antibody
For Western blots use StrepMAB-Classic HRP conjugate.
StrepMAB-Classic/-Immo fluorescent conjugatesFor fluorescent detection of Strep-tag¢ç proteins in
Available as single conjugates of 50µg for different wavelength:
StrepMAB Classic or StrepMAB Immo conjugated with Chromeo 488¢â
StrepMAB Classic or StrepMAB Immo conjugated with Chromeo 542
StrepMAB Classic or StrepMAB Immo conjugated with Oyster 645
Note, Fluorescent Detection Trial Kits were discontinued on December 31, 2013 and replaced by single fluorescent conjugates.
Secondary HRP antibodyRabbit anti Mouse pAb, HRP conjugated
Rabbit anti Mouse pAb, horseradish peroxidase (HRP) conjugatedRabbit anti Mouse pAb, HRP conjugated, is recommended for detection of
Strep-tag¢ç Protein LadderAccurate MW determination and positive control on Western blots!
Strep-tag¢ç Protein LadderAccurate MW determination and positive control on Western blots!The Strep-tag¢ç Protein Ladder(#2-1011-100) simplifies the comparison of Coomassie stained gels with corresponding Western blots. Thus, Strep-tag¢ç fusion proteins can be located accurately in complex band patterns on Coomassie gels.
The Strep-tag¢ç Protein Ladder is a mixture of six recombinant, highly purified Strep-tag¢ç proteins employed for precise sizing of proteins by SDS-PAGE.
The proteins resolve into clearly identifiable sharp and evenly stained bands from 15 to 100 kDa when analyzed on an SDS gel and stained with Coomassie Blue.
As each protein contains the Strep-tag¢ç II sequence which is detected by Strep-Tactin¢ç conjugates or Strep-tag¢ç specific antibodies, the ladder can also be used for MW determinations on Western blots and serves as a positive control for the various detection systems.
|
Purification | purified by affinity chromatography |
Host animal/Subclass | Mouse/IgG1 |
Specificity | N- or C-terminal or internal Strep-tag¢ç II |
Form | lyophilized from NH4HCO3 with 10 mg sucrose per mg antibod |
Stability | 18 months after shipment |
Storage | stored at -20 ¡ÆC |
Shipment | RT |
Purification |
purified by affinity chromatography |
HRP conjugate |
conjugated to 3-3.5 mols horseradish peroxidase per mol IgG |
Host animal/Subclass |
Mouse/IgG1 |
Specificity |
N- or C-terminal or internal Strep-tag¢ç II |
Form |
suspension in PBS with 0.5 % BSA and 0.01 % thimerosal |
Stability |
18 months after shipment |
Storage |
stored at 4 ¡ÆC, do not freeze! |
Shipment |
RT |
Host animal/Subclass |
Mouse/IgG1 |
Specifity |
Nearly irreversible binding of C- or N-terminally fused Strep-tag¢ç II, which is N-terminally extended by "SerAla" ** like recombinant protein - SA-WSHPQFEK or SA-WSHPQFEK - recombinant protein. Proteins containing Strep-tag¢ç II with N-terminal sequences other than SerAla are bound with reduced affinity in the cases tested so far. |
Form binding capacity |
lyophilized from NH4HCO3 with 10 mg sucrose per mg antibody (determined for bacterial alkaline phosphatase) |
Stability |
24 months after shipment |
Storage |
-20 ¡ÆC |
Shipment |
RT |
Description |
Purified polyclonal rabbit anti mouse immunoglobulin fraction conjugated to horseradish peroxidase (HRP). |
Specifity |
The antibody used for conjugation has not been absorbed to remove cross-reactions to immunoglobulin from other species. Therefore the conjugate shows reactions with mouse immunoglobulin of all classes |
Form |
Dissolved in 50 mM Tris/HCl 15 mM NaN3, pH 7.2 |
Concentration of conjugate |
1.3 mg/ml |
Amount |
0.5 ml |
Dilution |
|
Storage |
2 - 8 ¡ÆC |
Stability |
6 months after shipping |
Shipment |
RT |