Staining of antigen-specific MHC I T cells with reversible MHC I StreptamersThe MHC I Streptamers, adducts of Strep-tagged MHC I molecules (MHC I-Streps) and Strep-Tactin¢ç, are directed to antigen-specific CD8 T cell receptors.
Cell staining procedure1) Peptide (antigen)-loaded MHC I-Streps (Strep-tagged MHC I molecules) are multimerized with Strep-Tactin¢ç attached to a fluorochrome to generate multimers with high binding avidity. These multimers are determined for staining of T cells which are specific for the respective peptide-loaded MHC molecule. (Fig.1).
Fig. 1: MHC I Streptamer¢ç Fluorochrome
2) After FACS analysis or FACS sorting of the target cells, the staining reagents can be completely removed with biotin (Fig.2). Cells are left phenotypically and functionally indistinguishable from untreated cells.
Fig.2: Antigen-specific cytotoxic CD8 T cells are selected by a MHC I Streptamer¢ç. After staining and fluorescence assisted sorting, the Strep-Tactin¢ç complex is removed from the target cells by the addition of biotin under physiological conditions at low temperatures (4¡ÆC). The monomeric low affinity MHC I-Strep molecules are then dissociated completely from the cell surface by two short washing steps. The isolated cells are phenotypically and functionally undistinguishable from non-treated cells and predestined for clinical use. Fluorescent MHC I Streptamers for antigen-specific staining of (CD8+) T cells with reversible reagentsFluorescent MHC I Streptamers comprise a MHC I-Strep which has to be associated with a Strep-Tactin¢ç fluorochrome in a short incubation step. The resulting Streptamers are then used for antigen-specific staining of the target cells. After staining and FACS sorting, the Streptamers can be completely removed from the cells with biotin (stock solution included in the Streptamer¢ç Solution Set Standard for washing and dissociation).
Modular Streptamer¢ç technology : the MHC I-Streps can be associated either with a Strep-Tactin¢ç fluorochrome for cell staining or with a Strep-Tactin¢ç Magnetic Bead for cell isolation.
single MHC I Streptamer¢ç products for cell staining
*For larger amounts please refer to the product list or inquire at info@streptamer.com
Best CELLer Staining KitsAssemble your Best CELLer Staining Kit in two steps:
Please use filters "Category" and "Subcategory" on top of the product list
* 1 test is for staining of 1x10^6 cells.
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