SensiMix SYBR¢ç No-ROX kit contains all the components necessary to perform consistent and reproducible real-time PCR assays, but does not contain a passive reference and so is suitable for those block-based and rotor-based machines where an internal reference is not required for normalization. The mix has been developed to provide the high sensitivity required for demanding assays or for those assays where template is limited.
Features & BenefitsSensitivity - allows reproducible detection of low-copy number templatesReproducibility - generates real-time data reliably and reproducibly
Specific - proprietary hot-start chemistry minimises non-specific amplification
Broad dynamic range - a dynamic range of up to eight orders of magnitude Instrument CompatibilityRoche LightCycler¢ç 480, LightCycler¢ç Nano, Bio-Rad Opticon¢â, Opticon¢â2, Chromo4¢â, MiniOpticon¢â, CFX96¢â, CFX384¢â, Cepheid SmartCycler¢ç, Qiagen Rotor-Gene¢â 3000, Rotor-Gene¢â 6000, Rotor-Gene¢â Q, Analytik Jena qTOWER 2, Eppendorf Mastercycler¢çep realplex, Illumina¢ç Eco¢â, Takara Thermal Cycler Dice¢ç (TP800) and Techne Quantica¢ç, PrimeQ (See product selection table). DescriptionSensiMix SYBR¢ç No-ROX kit is a premixed high performance product designed for superior sensitivity and specificity on various real-time instruments in which a ROX signal is not required for normalization. Powered by a proprietary highly optimized chemical hot-start PCR enzyme, the mix has been developed to provide the high sensitivity and specificity (fig. 1) required for demanding assays or for those assays where template is limited.
SensiMix SYBR¢ç Low-ROX is provided as a 2x mastermix containing all the components necessary for real-time PCR, including the SYBR¢ç Green I dye, dNTPs, stabilisers and enhancers. NotesSensiMix is a trademark of Bioline Reagents Ltd. protocol [pi_50233_sensimix_sybr_no_rox_kit_v1.pdf] (395,325kb) |
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