Molecular BeaconsMolecular BeaconsÀº ÀÚ¿¬»óÅ¿¡¼ stem-loop(hair-pin)À» Çü¼ºÇÒ ¼ö ÀÖµµ·Ï °í¾ÈµÈ self-complementary end¸¦ °¡Áø Ưº°ÇÑ dual-label probeÀÔ´Ï´Ù. ÀÌ hairpinÀº reporter¿Í quencher¸¦ ´õ¿í °¡±îÀÌ µÎ±â À§ÇÑ ±¸Á¶À̸ç target¿¡ hybridizationÇÏ¿© reporter¿Í quencher°¡
¸Ö¸® ºÐ¸®µÇ¸ç Molecular BeaconsÀÌ ºûÀ» ³»°Ô µË´Ï´Ù. ÀÌ probesÀÇ hairpinsÀ¸·Î ÀÎÇØ target¿¡ ´ëÇÑ specificity´Â °ÈµÉ ¼ö ÀÖÀ¸¸ç Molecular Beacons Àº simple linear probesº¸´Ù SNPs¸¦ ´õ¿í Àß ÆÇ º°ÇÒ ¼ö ÀÖ½À´Ï´Ù.
Molecular Beacons´Â hybridizationÀ» ÅëÇØ »óº¸ÀûÀÎ nucleic acid¸¦ detectionÇÏ´Â °¡Àå º¸ÆíÀûÀÎ ¹æ¹ýÀÌ¸ç ¶ÇÇÑ triplex DNA formation ÀÇ thermodynamics¿¡¼µµ ¸¹ÀÌ »ç¿ëµÇ±âµµ ÇÕ´Ï´Ù.
Molecular Beacon º¸Àå·®
45 bases ±îÁöÀÇ ¿Ã¸®°í¿Í , reporter, quencher, HPLC purification, QC measurement of signal-to-noise ratio (see below)°¡ Æ÷ÇԵǾî ÀÖ½À´Ï´Ù. ±æÀÌ°¡ 45 baseÀÌ»óÀÎ Molecular BeaconsÀº º°µµ·Î ¹®ÀÇÇϽñ⠹ٶø´Ï´Ù.
TYE¢â563Àº Cy3°ú ÆÄÀåÀÌ °°°í TYE¢â665´Â Cy5¿Í ÆÄÀåÀÌ °°½À´Ï´Ù.
|
»ç¿ëÈıâ ÀÛ¼º
|