"After getting nowhere with other kits we tried the SensiFAST SYBR One-Step Kit and saw results first time, I cannot describe how wonderful this made us feel."
- Asia-Pacific Special Nutrients Sdn. Bhd.
Features and Benefits• ¹Î°¨¼º - ±¤¹üÀ§ÇÑ À¯ÀüÀÚ ¹ßÇöÀÇ Á¤È®ÇÑ Á¤·® • ºü¸§ - ÃÖ°íÀÇ Æ¯À̼º°ú ¹Î°¨µµ¸¦ À§ÇØ µ¶Æ¯ÇÑ ¹öÆÛ »ç¿ë • Bio-Rad ±â°è¿Í ȣȯ°¡´É - dynamic well factor¸¦ °è»êÇϱâ À§ÇØ flouresceinÀÌ ÇÊ¿äÇÑ ±â°è¿¡¼ »ç¿ë (SensiFAST SYBR & Fluorescein kit)
• Æí¸®¼º - ºü¸£°í ½¬¿î one-tube ¼Â¾÷ • Protocol - fast qPCR °ú standard qPCR protocol·Î ¸ðµÎ »ç¿ë °¡´É Instrument CompatibilityABI 7000, 7300, 7700, 7900, 7900HT, StepOne¢â, StepOne¢â Plus, each of these instruments having the capacity to analyze the real-time PCR data with the passive reference signal either on or off, as well as several instruments that do not require the use of ROX. DescriptionSensiFAST SYBR One-step Kits´Â ºü¸£°í RT-qPCRÀ» À§ÇØ °³¹ßµÇ¾úÀ¸¸ç ÀϹÝÀûÀ¸·Î »ç¿ëµÇ°í ÀÖ´Â real-time ±â°è¿¡¼ °ËÁõÀÌ µÇ¾ú½À´Ï´Ù. SensiFAST SYBR One-Step Kits´Â single tube¿¡¼ ³ôÀº Àç»ý¼ºÀÇ first strand cDNA¿Í ÈÄ¼Ó real-time PCRÀÌ °¡´ÉÇϵµ·Ï Á¶¼ºµÇ¾ú½À´Ï´Ù(fig 1). ÃÖ½ÅÀÇ buffer Á¶¼º°ú enhancer ±×¸®°í antibody ±â¹Ý hot-start DNA PolymeraseÀÇ Á¶ÇÕÀº SensiFAST SYBR kit°¡ ºü¸£°í ³ôÀº Àç»ýÀ²°ú ÃÖ°í·Î ¹Î°¨ÇÑ real-time PCR(fig 2)ÀÌ °¡´ÉÇϵµ·Ï ÇÕ´Ï´Ù. ÀÌ kit´Â DNA template¿¡ ÀûÇÕÇÏ°í RNA templateÀº Bioline Tetro cDNA Synthesis Kit(BIO-65042)·Î revere transcriptionÇÑ ÈÄ »ç¿ë °¡´ÉÇÕ´Ï´Ù. SensiFAST One-step kit´Â 2X SensiFAST SYBR One-Step mix¿Í º°µµÀÇ reverse transcriptase, RiboSafe Inhibitor ·Î ±¸¼ºµÇ¾î ÀÖ½À´Ï´Ù. SensiFAST SYBR Hi-Rox kit´Â ¼±Åûç¿ëÀ» À§ÇØ premixed ROX°¡ Æ÷ÇԵǾî ÀÖ½À´Ï´Ù. VolumeBIO-73001: 100 x 20µl Reactions: 1 x 1ml BIO-73005: 500 x 20µl Reactions: 5 x 1ml Components2x Reaction mix consisting of: Heat-activated DNA polymerase
Ultra-pure dNTPs
MgCl2
ROX
SYBR¢ç Green I Plus separate vials of: Reverse transcriptase, RNase inhibitor, DEPC-treated water
Kit SizeThe kit size is based on a 20µl final reaction volume. Storage Conditions12 months at -20¡ÆC. Avoid exposure of the SYBR¢ç Green I to light. Product CitationsBergland A.O. et al. PLoS Genet. 8(4): e1002631(2012) NotesSensiFAST is a trademark of Bioline Reagents Ltd. protocol [pi_50201_sensifast_probe_hi_rox_one_step_kit_v1.pdf] (442,164kb) |
»ç¿ëÈıâ ÀÛ¼º
|