Probe-Based qPCR Master Mix Ãâ½ÃPrimeTime¢ç Gene Expression Master Mix ´Â probe-based qPCR assays¿¡ ÃÖÀûÈ µÇ¾î ÀÖ°í, two-step RT-qPCR ¿¡¼ >90% ÀÇ È¿À²À» º¸Àå ÇÕ´Ï´Ù.
Consist of : As good or better than other commercial master mixes PrimeTime¢ç Gene Expression Master Mix delivers consistent results that are as good or better than the other commercially available master mixes tested. Cq values using the IDT master mix were approximately 2–4 Cqs earlier in this representative experiment. Figure 1. PrimeTime¢ç Gene Expression Master Mix delivers consistent results that are as good or better than 7 other commercially available master mixes tested. PCRs contained PrimeTime HPRT qPCR Assays, PrimeTime Gene Expression Master Mix with reference dye, and either cDNA or gBlocks¢ç Gene Fragments as template. PCRs were run in triplicate on a 7900HT Real-Time PCR System (Thermo Fisher Scientific).
PrimeTime¢ç Gene Expression Master Mix performs well under standard or fast cycling conditions (Figures 1–2) on a variety of real-time qPCR instruments, including the 7900HT Real-Time (Thermo Fisher Scientific), QuantStudio¢â 7 Flex (Thermo Fisher Scientific), CFX384 (BioRad), and LightCycler¢ç 480 (Roche) qPCR systems. Figure 4. High PCR efficiency under standard or fast cycling conditions. PCRs consisting of PrimeTime¢ç qPCR Assays, PrimeTime Gene Expression Master Mix, reference dye, and template were run on a 7900HT Real-Time PCR System (Thermo Fisher Scientific) using standard or fast cycling conditions. Standard cycling conditions: 3 min. 95¡ÆC; 49 x (15 sec. 95¡ÆC; 1 min. 60¡Æ). Fast cycling conditions: 3 min. 95¡ÆC; 49 x (5 sec. 95¡ÆC; 30 sec. 60¡Æ). This histogram shows the calculated PCR efficiency of 13 assays run under standard (orange) or fast (blue) cycling conditions using either diluted cDNA (0.016–50 ng) or gBlocks¢ç Gene Fragments (101–107 copies) as template. All assays exhibited 90–110% PCR efficiency with R2 >0.99. PrimeTime_qPCR_Application_Guide,_4th_Ed..pdf (12,485,387kb) |
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