StepView¢â 10kD Prestained Protein Marker10 kD Prestained Protein marker´Â 11°³ÀÇ recombinant proteinsÀÌ ¾à 10 kDa ¾¿ Â÷À̸¦ µÎ°í ÀÖ´Â protein size marker·Î 15~120 kDa »çÀÌÁîÀÇ ´Ü¹éÁúÀ» È®ÀÎ Çϱ⿡ ÀûÇÕÇÑ marker ÀÔ´Ï´Ù. Pre-stained marker À̹ǷΠÀü±â¿µµ¿ÇÏ¸é¼ ÇÔ²² È®ÀÎ °¡´ÉÇÕ´Ï´Ù.
Features• Dual color size standard (blue and pink)
• 10 Kda size increment (easy size estimation) • Highly purified and blended 11 recombinant proteins • His-tag sequence in the proteins enables direct detection on western blots using anti-his antibody • User oriented ready to use solution DescriptionMbiotech's Step-view¢â 10Kd prestained protein molecular weight standard is a
dual color prestained size marker. It is a mixture of 11 recombinant purified
and prestained polypeptides whose molecular weights are well-adjusted.
Step-viewTM 10Kd prestained protein molecular weight standard is available in a
ready-to-use formula (no need to boil before use), and is suitable for sizing
proteins between 15,000 and 120,000 Da by SDS-PAGE. A blue and pink chromophore
is covalently bound to recombinant proteins, and 11 prestained proteins are
readily visible during electrophoresis or electrophoretic transfer from the gel
to a membrane. The 50 and 100 Kda proteins labeled with pink chromophore enables
easy discrimination. Because coupling of chromophore to the proteins affects
their apparent molecular weights in SDS-PAGE relative to unstained proteins,
unstained protein standard (Cat. No. 20011) should be used for accuracy >95%.
Recommended storage condition• -20¡É for one year
• Please
aliquot in a small volume for
prevention of repeated freezing & thawing
Usage information ¢Â Recommended loading
volume:
5µl/lane for mini gel(10x8cm, 0.75 or
1mm thick)
10-15µl/lane for large gel(30x20cm, 1
or 1.5mm thick)
¢Â Typical number of
lanes: 50
¢Â Number of Bands: 11
¢Â Range (KDa): 15-120
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