LandMark¢â Broad Range Prestained Protein Marker(all blue)DescriptionLandMarkTM broad range prestained protein marker(all
blue) contains 10 blue colored polypeptides whose molecular weights are
well-adjusted ranging from 7,000 to 240,000 Da. It is provided preblended in a
ready-to-use formula and no reconstitution or further dilution is necessary
before use. A blue chromophore is covalently bound to proteins, and 10 prestained
proteins are visible during electrophoresis or electrophoretic transfer from
the gel to a membrane. The 25 KDa and 70 KDa proteins serve as a landmark
because they are present at a higher concentration in the mixture relative to
the adjacent bands. Because coupling of chromophore to the proteins affects
their apparent molecular weights in SDS-PAGE, unstained protein standard should
be used for accuracy >95%.
Features• Blue dyed proteins with two contrasting bands
• Broad range size estimation(7-240kDa) • Easy visualization during electrophoresis and evaluation of western transfer efficiency • User oriented ready to use solution Recommended storage condition• -20¡É for one year
• Please
aliquot in a small volume for
prevention of repeated freezing & thawing
Usage information¢Â Recommended loading
volume:
5µl/lane for mini gel(10x8cm, 0.75 or
1mm thick)
10-15µl/lane for large gel(30x20cm, 1
or 1.5mm thick)
¢Â Typical number of
lanes: 100
¢Â Number of Bands: 10
¢Â Range (KDa): 7-240
10% Gradient Gel, 5 ul loading data |
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