Custom DNA Oligos

DNA ռ

 
Base Pricing
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25 nmole(15-60 Bases)
100 nmole(10-90 Bases)
250 nmole(5-100 Bases)
1 mole(5-100 Bases)
5 mole(5-100 Bases)
10 mole(5-100 Bases)
 
 

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Product

Available length

Guaranteed yield*

Standard desalt

HPLC purification

PAGE purification

Custom DNA

25 nmol DNA oligo

15–60 bases

10 nmol

N/A

N/A

100 nmol DNA oligo

10–90 bases

30 nmol

3.75 nmol

2 nmol

250 nmol DNA oligo

5–100 bases

50 nmol

12.5 nmol

6.5 nmol

1 µmol DNA oligo

5–100 bases

200 nmol

50 nmol

25 nmol

5 µmol DNA oligo

5–100 bases

1000 nmol

250 nmol

125 nmol

10 µmol DNA oligo

5–100 bases

2000 nmol

500 nmol

250 nmol

Custom RNA

100 nmol RNA oligo

5–60 bases

15 nmol

2 nmol

N/A

250 nmol RNA oligo

5–60 bases

30 nmol

7.5 nmol

1 µmol RNA oligo

5–60 bases

100 nmol

25 nmol

5 µmol RNA oligo

5–60 bases

500 nmol

125 nmol

10 µmol RNA oligo

5–60 bases

1000 nmol

250 nmol

* Guaranteed yield for unmodified oligos 25-50 bases; will vary with oligo composition, length, and purification.


Standard DNA ռ ¾ ϴ.
ռ 2~4 ҿǸ 5 umole ̻ 1 ҿ ˴ϴ.
Large-scale ռ (up to 2 grams) մϴ. ٶϴ.
Cloning̳ sequencing 뵵 ø õմϴ.
ø(200mer) ռ Ultramer Ͻñ ٶϴ.
ٷο ռ ռ ̵ level II setup (100,000~270,000) ߰ ֽϴ. 
* 差 ֽϴ.

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LabReady Service Fee
LabReady Fee

RxnReady Mixing Service Fee
25 nm, 100 nm and 250 nm Mixed Oligos

Normalized Tube Fee

25 nm, 100 nm, 250 nm and 1 um DNA Oligos

5 um DNA Oligo

10 um DNA Oligo

 Machine Mix Base  
 Hand Mix Base  
 
 

DNA Oligonucleotide Resuspension and Storage

 
IDT recommends resuspension in TE buffer rather than water. Oligonucleotides resuspended in water will have less stability and will have to be shipped on dry ice, which is more costly than standard shipping. Stability studies at IDT have shown that oligonucleotides stored in water at room temperature may show some degradation in as little as 60 days. The same studies showed that oligonucleotides stored dry or in TE at room temperature did not show signs of degradation for at least 7 months.
 

Starting Scale and Yield

 
IDT uses a starting scale, referred to as just scale, for the amount of initiating nucleotide used to begin oligo synthesis. However, processing steps such as cleavage and deprotection of the oligonucleotide will reduce the final delivery amount (yield). The addition of modifications, purification and length combinations also all affect the final yield of each oligo, which will always be less than the starting scale. The minimum yield guarantee is given in the shopping cart and in the order confirmation email. To receive more final yield, select a larger synthesis scale.
 

Purification and Yield

 
Additional purification significantly improves oligonucleotide performance in demanding applications such as multiplex PCR, cloning, mutagenesis, and antisense/RNAi methods. However, purification will always lower oligonucleotide final yield. HPLC routinely provides 85% purity, while PAGE provides 90% purity. The final yield of a PAGE purified oligo, however, will be less than an HPLC purified oligo. For example, a 250 nmole scale 25mer oligo will yield 8X with standard desalt, but only 2X with HPLC purification and 1X with Page purification.
 

Tools for Oligonucleotide Analysis and Design

 
IDT provides a number of free oligonucleotide design and analysis tools in the online SciTools suite. The suite is comprised of tools for designing assay components as well as determining the properties of any sequence entered. Check out the SciTools Suite website at www.idtdna.com/scitools
 

IDT Guarantees Quality

 
IDT has pioneered the use of high throughput Quality Control methods and is the only oligonucleotide manufacturer that offers 100% QC and purity guarantees. Today, every oligo synthesized at IDT undergoes QC by mass spectrometry. IDT also offers CE and analytical HPLC as routine tools for additional QC on purified oligos. In addition to DNA, IDT has led the effort over the last decade to reduce the cost of high-quality custom RNA synthesis. Through improvements to the traditional tert-butyldimethylsilyl (tBDMS) chemistry and advances in instrumentation, IDT has achieved the highest coupling efficiency in the industry. Only at IDT are customers guaranteed the very best in quality synthesis with free mass spectrometry and CE traces on all purified oligos.
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IDT oligo
IDT oligo
IDT ø
oligo dT primer
IDT oligomer
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SensiFAST SYBR Hi-ROX Kit
SensiFAST SYBR Lo-ROX Kit
MyTaq DNA Polymerase
MyTaq Mix
MyTaq Red DNA Polymerase
MyTaq Red Mix
SensiFAST SYBR No-ROX Kit
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