The Genomic DNA purification kit (for tissue sample) is the ideal tools for isolation and purification of genomic DNA from fresh or frozen human and animal tissues, biopsy material, rodent tail, insects and paraffin embedded tissues, as well as animal origin food samples and eukaryotic cells, and cell pellets. For reproducible and high yields appropriate sample storage is essential. The purified DNA can be used for molecular biological analysis.

The product is intended for use by professional users such as technicians, physicians and biologists trained in molecular biological techniques. It is designed to be used with any downstream application employing enzymatic amplification or other enzymatic modification of DNA followed by signal detection or amplification. Any molecular biological results generated using the sample preparation procedure in conjunction with any downstream molecular biological assay should be interpreted with regard to other clinical or laboratory findings. To minimize irregularities in molecular biological results, adequate controls for downstream applications should be used.

 

The Genomic DNA purification kit (for tissue sample) is an excellent tool offering a speedy and economic method to purify total DNA from several types of animal tissue. Some specially modified protocols are developed for other samples, such as bacterial, fixed tissue, yeast etc. This technology first lyses cells and degrades protein by using a chaotropic salt and Proteinase K, then binds DNA to silica based membranes, washes DNA with ethanol contained wash buffer and then elutes purified DNA by low salt elution buffer or ddH₂O. Compare with other harmful and time-consuming procedures, this kit shortens the handling time within 1 hour (lysis time varies depending on the type of tissue processed). The size of purified DNA is up to 50kb (predominantly 20 - 30kb). DNA of this length performs the highest efficiency during PCR reaction. After using this kit, the high quality total DNA can be used directly for the down-stream applications.

 

Genomic DNA Purification Kit (for tissue sample)
Product	50 preps	200 preps	Storage
Cat. No.	16052	16202	Condition
TG I Buffer	10 ml	40 ml	RT
TG II Buffer	11 ml	44 ml	RT
Wash Buffer I (conc.)	16 ml	33 ml X 2	RT
Wash Buffer II (conc.)	11 ml	22 ml X 2	RT
Elution Buffer	15 ml	23 ml	RT
Proteinase K	1 ml	1 ml X 4	4 ¡É
Column	50	200	RT
2.0 ml Collection Tube	50	200	RT
Protocol	1	1	-

 

The performance of Genomic DNA Purification Kit (for tissue sample) is monitored routinely on a lot number. Genomic DNA Purification Kit (for tissue sample) can be guaranteed the correct function for applications as described in the manual. In accordance with Mbiotech¡¯s certified Quality Management System each component of the Genomic DNA Purification Kit (for tissue sample) was tested against predetermined specifications to ensure consistent product quality. The quality of isolated DNA is checked by restriction digestion, agarose gel electrophoresis, and spectrophotometry.

 

Storage

Storage Condition