The Plasmid MIDIprep kit has been optimized for the rapid purification of DNA up to 60 ml of bacteria for high-copy number plasmid(up to 120ml of bacteria for low-copy number plasmid).

This kit provides the alkaline lysis reagents and the columns packed with anion-exchanger resin. After the cells lysis, the plasmid DNA is bound to the resin insided the column by a gravity-flow procedure, and the contaminants can be remove with wash buffer. After using this convenient kit, the purified plasmid DNA is suitable for downstream application such as transfection, in vitro transcription and translation, and all enzymatic modification. Column binding capacity is 200~300 ug/ column.

 

Plasmid DNA Midi Kit
Product	25 preps	50 preps	Storage
Cat. No.	11525	11550	Condition
Cell Resuspension Solution	63 ml	63 ml x 2	RT
Cell Lysis Solution	63 ml	123 ml	RT
Neutralization Solution	90 ml	120 ml + 53 ml	RT
Wash Buffer	25 ml x 2	46 ml x 2	RT
Elution Buffer	27 ml	53 ml	RT
RNase A (50mg/ml)	200 ul	200 ul x 2	4 ¡É
MIDI Column	25 ea	50 ea	RT
Protocol	1 ea	1 ea	-

 

Plasmid DNA Midi Kit
Sample Size : up to 60ml of bacteria for high-copy number plasmid 
                     up to 120ml of bacteria for low-copy number plasmid

Binding Capacity: 200~300 ug/ column

Isolation of high copy plasmid DNA
Isolation of low copy plasmid DNA

Kit contents should be stored dry at room temperature. Under these conditions, the kit stable for 12 months.

 

Room temperature.

 

The Plasmid MAXI prep kit has been optimized for the rapid purification of DNA up to 60-240 ml of bacteria for high-copy number plasmid(up to 200-480 ml of bacteria for low-copy number plasmid).

This kit provides the alkaline lysis reagents and the columns packed with anion-exchanger resin. After the cells lysis, the plasmid DNA is bound to the resin insided the column by a gravity-flow procedure, and the contaminants can be remove with wash buffer. After using this convenient kit, the purified plasmid DNA is suitable for downstream application such as trasfection, in vitro transcription and translation, and all enzymatic modification.

Column binding capacity is 500~600 ug/ column.

 

Plasmid DNA MAXI Kit
Product	10 preps	20 preps	Storage
Cat. No.	11610	11620	Condition
Cell Resuspension Solution	43 ml x 2	55 ml x 3	RT
Cell Lysis Solution	84 ml	165 ml	RT
Neutralization Solution	115 ml	230 ml	RT
Wash Buffer	12 ml + 25 ml	25 ml x 3	RT
Elution Buffer	15 ml	25 ml	RT
RNase A (50mg/ml)	200 ul x 2	200 ul x 3	4 ¡É
MAXI Column	10 ea	20 ea	RT
Protocol	1 ea	1 ea	-

 

The performance of Plasmid MAXI prep Kit is monitored routinely on a lot number. This kit is tested with high-copy plasmid DNA from 40 ml overnight culture in LB medium and yield of plasmid DNA is measured with spectrophotometer. The quality of isolated DNA is checked by restriction digestion, agarose gel electrophoresis, and spectrophotometry.

 

Plasmid DNA Maxi Kit
Sample Size : up to 60 -240ml of bacteria for high-copy number plasmid 
                   up to 200 -480ml of bacteria for low-copy number plasmid

Binding Capacity: 500~600 ug/ column

Isolation of high copy plasmid DNA
Isolation of low copy plasmid DNA

Kit contents should be stored dry at room temperature. Under these conditions, the kit stable for 12 months.

Room temperature.