The RNA Mini Purification Kit (for tissue sample) is the ideal tool for reliable and fast manual isolation and purification of high quality total RNA from animal tissue and cultured cells. For reproducible high yields and appropriate sample storage and quick operation under the rules for RNA operation is essential. The purified RNA is ready to use for molecular biological analysis. The isolation protocol and all buffers are optimized to assure a high yield as well as a high purity of purified total RNA. All manual work is reduced to a minimum.

The product is indented for use by professional users such as technicians, physicians and biologist trained in molecular biological techniques. It is designed to be used with any downstream application employing enzymatic amplification or other enzymatic modifications of RNA followed by signal detection or amplification.

 

This method first lyses cells by using a chaotropic salt, then binds RNA to silica-based membranes, washed RNA with ethanol-contained wash buffer and then elutes purified RNA by RNase-free ddH2O. It takes 30 min for an entire procedure, and the purified RNA is ready for RT-PCR, northern blotting, primer extension and cDNA library construction.

 

Sampling: up to 30 mg tissue             Up to 1 X 107 animal cultured cells, 1 X 109 bacterial cells, or 5 X 107 yeast Yield: Up to 40 ¥ìg total RNA Handling time: about 30 minutes

 

RNA Mini Purification Kit
Product	50 preps	200 preps	Storage
Cat. No.	15300	15400	Condition
TRB Buffer	25 ml	100 ml	RT
Wash Buffer I	30 ml	120 ml	RT
Wash Buffer II (conc.)	15 ml	35 ml x 2	RT
RNase-free ddH2O	6 ml	12 ml	RT
Filter Column	50 ea	200 ea	RT
Column	50 ea	200 ea	RT
2.0ml Collection Tube	100 ea	400 ea	RT
Elution Tube	50 ea	200 ea	RT
Micropestle	50 ea	200 ea	RT
Quick Guide	1 ea	1 ea	-

 

RNA Mini Purification Kit can be guaranteed the correct function for applications as described in the manual. In accordance with Mbiotech¡¯s certified Quality Management System each component of the RNA Mini Purification Kit were tested against predetermined specifications to ensure consistent product quality.

 

All buffers and kit contents should be stored at room temperature (RT) and are stable for at least 12 months under these conditions.

Room temperature is defined as range from 15-25¡ÆC.

 

Sample	Recommended amount of sample used	RNA yield(ug)
Animal cells (up to 5 x 106)	NIH/3T3 HeLa COS-7 LMH	1x106 cells 1x106 cells 1x106 cells 1x106 cells	10 15 30 12
Animal Tissues (Mouse/rat) (up to 30 mg)	Embryo Heart Brain Kidney Liver Spleen Lung Thymus	10 mg 10 mg 10 mg 10 mg 10 mg 10 mg 10 mg 10 mg	25 10 10 30 50 35 15 45
Bacteria	E.coli B.subtilis	1x109cells 1x109 cells	60 40
Yeast (up to 5x107)	S.cerevisiae	1x107 cells	25