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- Plasmid Miniprep kit
- plasmid Midiprep/ Maxiprep Kit
- PCR Purification Kit
- Gel Extraction Kit
- Genomic DNA purification Kit (Blood sample)
- Genomic DNA purification Kit (Tissue sample)
- Genomic DNA purification Kit (Bacterial sample)
- Total RNA Miniprep Kit (Blood, bacteria and cultured cells sample)
- Total RNA Miniprep Kit (Animal tissue sample)
- Total RNA Isolation Solution
- GoPhorIT™ Taq DNA Polymerase
- ExAct™ Taq DNA Polymerase
- Hiphi™ DNA Polymerase
- Hot Taq DNA Polymerase
- 2X PCR Mastermixes
- 2X PCR Premixes
- OneStep RT-PCR Premix
- qPCR SYBR Green 2X Mastermix Kit
- qPCR SYBR Green 2X Mastermix Kit with Low-ROX
- One-Step qPCR SYBR Green 2X Mastermix Kit
- One-Step qPCR SYBR Green 2X Mastermix Kit with Low-ROX
- qPCR Probe 2X Mastermix Kit
- One Step qPCR Probe 2X Mastermix Kit
- dNTPs
- Protein markers
- DNA Marker
- Agarose
- Acrylamide stock solutions
- Ready to Use Electrophoresis Buffers
- SeeNA™ Nucleice Acid Stains
qPCR SYBR Green 2X Mastermix Kit with Low-ROX
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18500
18500 Description
The Mbiotech SYBR Low-ROX Kit is a high-performance reagent designed for superior sensitivity and specificity on various real-time instruments. The Mbiotech SYBR Low-ROX Kit employs a hot-start DNA polymerase, for high PCR specificity and sensitivity. Mbiotech SYBR Low-ROX Kit is inactivate and possesses no polymerase activity during the reaction set-up, preventing non-specific amplification including primer-dimer formation.
For ease-of-use and added convenience, Mbiotech SYBR Low-ROX is provided as a 2x mastermix containing all the components necessary for real-time PCR, including the SYBR® Green I dye, dNTPs, stabilizers and ROX for optional use (see notice to purchaser No. 5 in Trademark and Licensing Information). As a ready-to-use premix, only primers and template need to be added.
For ease-of-use and added convenience, Mbiotech SYBR Low-ROX is provided as a 2x mastermix containing all the components necessary for real-time PCR, including the SYBR® Green I dye, dNTPs, stabilizers and ROX for optional use (see notice to purchaser No. 5 in Trademark and Licensing Information). As a ready-to-use premix, only primers and template need to be added.
Storage and Stability
The Mbiotech SYBR Low-ROX Kit is shipped on Dry/Blue Ice. All kit components should be stored at -20℃ upon receipt. Excessive freeze/thawing is not recommended. Since SYBR® Green I is light-sensitive, it is important to avoid prolonged exposure to light. When stored under optimum conditions, the reagents are stable for a minimum of 6 months from date of purchase.
Quality Control
The Mbiotech SYBR Low-ROX Kit and its components are extensively tested for activity, processivity, efficiency, heat activation, sensitivity, absence of nuclease contamination and absence of nucleic acid contamination prior to release.
Safety Precautions
Harmful if swallowed. Irritating to eyes, respiratory system and skin. Please refer to the material safety data sheet for further information.
Kit compatibility
The Mbiotech SYBR Low-ROX Kit is optimized for use on the real-time instruments listed in the following compatibility
table. However the kit is also compatible with several instruments that do not require the use of ROX, such as the Qiagen (Corbett) Rotor-GeneTM 6000, the Bio-Rad CFX96 or the Roche LightCycler® 480.
Optionl ROX: The Mbiotech SYBR ROX Kit is premixed with ROX (5-carboxy-X-rhodamine, succinymidyl ester), so that where necessary, ROX fluorescence can be optionally detected on certain real-time instruments. If your real-time instrument has the capability of using ROX and you wish to use this option, then this option must be selected by the user in the software (see notice to purchaser No. 6 in Trademark and Licensing Informtion).
table. However the kit is also compatible with several instruments that do not require the use of ROX, such as the Qiagen (Corbett) Rotor-GeneTM 6000, the Bio-Rad CFX96 or the Roche LightCycler® 480.
Optionl ROX: The Mbiotech SYBR ROX Kit is premixed with ROX (5-carboxy-X-rhodamine, succinymidyl ester), so that where necessary, ROX fluorescence can be optionally detected on certain real-time instruments. If your real-time instrument has the capability of using ROX and you wish to use this option, then this option must be selected by the user in the software (see notice to purchaser No. 6 in Trademark and Licensing Informtion).
Kit contents
| qPCR SYBR Green 2X Mastermix Kit with Low ROX | 2 X 1.25 ml | 4 X 1.25 ml | 10 X 1.25 ml | 20 X 1.25 ml |