The Mbiotech Probe One Step Kit contains a ready-to-use  reagent for probe-based real-time RT-PCR for use on all real-time instruments. The kit has been formulated for use with probe-detection technology, including TaqMan¢ç, Scorpions¢ç and molecular beacon probe. The Mbiotech Probe One-Step Kit provides high-performance, reproducible first strand cDNA synthesis and subsequent real-time PCR assays, using a single protocol. Both cDNA synthesis and PCR are performed in a single tube using gene-specific primers and either total RNA or mRNA. The kit combines a reverse transcriptase and a hot-start DNA polymerase, for high specificity and sensitivity of the one-step RT-PCR. Mbiotech Probe One-Step Kit is inactivate and possesses no polymerase activity during the reaction set-up preventing non-specific amplification including primer-dimer formation.
 
For ease-of-use and added convenience Mbiotech Probe One-Step Kit is provided as a 2x mastermix containing all the components necessary for reverse transcription and real-time PCR including dNTPs and stabilizers. As a ready-to-use premix, only primers and template need to be added. In addition, Mbiotech Probe One-Step Kit includes the highly efficient RNase inhibitor, which prevents enzymatic degradation of the RNA. In addition a separate tube of ROX is provided for optional use (see notice to purchaser No. 4 in Trademark and Licensing Information).

The Mbiotech Probe One-Step Kit is shipped on Dry/Blue Ice. All kit components should be stored at -20¡ÆC upon receipt. Excessive freeze/thawing is not recommended. When stored under optimum conditions, the reagents are stable for a minimum of 6 months from date of purchase.

The Mbiotech Probe One-Step Kit and its components are extensively tested for activity, processivity, efficiency, heat activation, sensitivity, absence of nuclease contamination and absence of nucleic acid contamination.

Harmful if swallowed. Irritating to eyes, respiratory system and skin. Please refer to the material safety data sheet for further information.

When handling RNA it is important to use RNase-free plasticware and reagents. We also recommend performing RNA work in an isolated area. To help prevent any carry-over DNA contamination, we recommend that separate areas are maintained for PCR set-up, PCR amplification and any post-PCR gel analysis. It is essential that any tubes containing amplified PCR product are not opened in the PCR set-up area.

Probe One-Step (2X)	2 X 1.25 ml	4 X 1.25 ml	10 X 1.25 ml	20 X 1.25 ml
RNase Inhibitor (10U/ ul)	100 ul	200 ul	500 ul	2 X 500 ul
25 uM ROX dye	100 ul	200 ul	500 ul	2 X 500 ul
DEPC-H2O	1 X 1.8 ml	2 X 1.8 ml	4 X 1.8 ml	8 X 1.8 ml