MBP(maltose binding protein) Excellose¢ç contains MBP Excellose¢ç spin column, collectionTube, and stock solution.
Protocols 1. Vortex the MBP Excellose¢ç tube and pipet 0.5ml of the slurry to Spin Column, add 0.5 ml of Equilibration Buffer in the Spin Column, and centrifuge at 700 x g (approx. 2,000 rpm) for 2 min. 2. Discard the eluate, load up to 0.5 ml of cell lysate, centrifuge for 2 min, and collect the flow-through fraction. 3. Add 0.5 ml of Washing Buffer, centrifuge for 2 min, and collect the wash fraction. 4. Repeat step 3, if necessary. 5. Add 0.2 ml of Elution Buffer and centrifuge. 6. Collect the eluate.
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