MMLV Reverse Transcriptase
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Detail View
19500
M-MLV RT (Molony Murine Leukemia Virus Reverse Transcriptase) is an RNA-dependent DNA polymerase requiring a DNA primer and an RNA template to synthesize a complementary DNA strand. M-MLV RT has a weaker intrinsic RNase H activity than AMV RT (Avian Myeloblastosis Virus Reverse Transcriptase). Purified from an E. coli strain carrying a M-MLV RT overproducing plasmid.
Recombinant E. coli strain
200 U/㎕
20 mM Tris-HCl (pH 7.5), 200 mM NaCl, 1 mM EDTA, 1 mM DTT, 0.01 % (v/v) Nonidet P-40, 50 % Glycerol
250 mM Tricine-KOH (pH 8.9), 50 mM (NHl4)l2SO4, 100 mM KCl, 50 mM DTT, 15 mM MgCl2
-20℃
M-MLV RT perform superior result, efficiency, sensitivity, and linearity over a wide range of starting RNA amounts.
Activity, SDS-PAGE/purity, ds-DNase, RNase, endonuclease/nickase, first-strand cDNA synthesis
One unit incorporate 1 nmol of deoxynucleotide into acid-precipitable material in 10 min at 37℃ using poly(A)oligo(dT)12-18 as template-primer.
Single 71 kDa band was visible in SDS-PAGE.
First strand cDNA synthesis
Primer extension |
