MBiotech¡¯s 100bp DNA mass ladder marker is a mixture of specially designed double-stranded DNA fragments for determining both the exact size and the amount of PCR products and engineered DNA fragments after agarose or polyacrylamide gel electrophoresis. The 100bp DNA mass ladder marker consists of DNA fragments ranging in size from 100 to 1,000 bp in 100 bp increments, and additional 1,500 bp fragment. The 100bp DNA mass ladder marker is supplied in a ready-to-use format. This ladder can be stained with ethidium bromide or radio labeled at 5¢¥-ends. The 100bp DNA mass ladder marker is premixed with loading buffer.

 

Ready-to-use : 10 mM Tris-HCl, pH8.0, 1 mM EDTA, 5% Glycerol, 0.005%, Bromophenol Blue, and  0.005% Xylene Cyanol

 

-20¡É for 1 year
4¡É for 6 months
Room temperature (20-25¡É) for 2 months

 

Recommended loading : 5-10 ml (ready-to-use)
Range : 100 – 1,500 bp
Number of bands : 11

 

5 ul/lane ; 1% agarose in 0.5X TAE, stained with ethidium bromide