MBiotech¡¯s 100bp DNA ladder marker is a mixture of specially designed double-stranded DNA fragments for determining the exact size of PCR products and engineered DNA fragments. The 100bp DNA ladder marker consists of 11 DNA fragments ranging in size from 100 to 1,000 bp in 100 bp increments, and additional 1,500 bp fragment. For easy reference on agarose gels, the 500 bp and 1,000 bp are two to three times brighter than the other bands. The 100bp DNA ladder markers are supplied in a ready-to-use or concentrated format. This ladder can be stained with ethidium bromide or radio labeled at 5¢¥-ends. The 100bp DNA ladder marker is premixed with loading buffer. The ladders are not intended for use in quantitative analysis.

 

Ready-to-use : 10 mM Tris-HCl, pH8.0, 1 mM EDTA, 5% Glycerol, 0.005% Bomophenol Blue, and 0.005% Xylene Cyanol

 

-20¡É for 2 years
4¡É for 6 months
Room temperature (20-25¡É) for 2 months

 

Concentration : 200 ng/5 ul (40 mg/ml)
Recommended loading : 5-10 ul (100-200 lanes, ready-to-use)
Range : 100 – 1,500 bp
Number of bands : 11

 

5 ul/200 ng/lane ; 1% agarose in 0.5x TAE, stained with ethidium bromide