MBiotech¡¯s 1Kbp DNA ladder marker is a mixture of specially designed double-stranded DNA fragments for determining the exact size of DNA fragments. The 1Kbp DNA ladder marker consists of 9 DNA fragments ranging in size from 500 to 10,000 bp. The 1Kbp DNA ladder marker is supplied in a ready-to-use format. This ladder can be stained with ethidium bromide or radio labeled at 5¢¥-ends. DNA ladder marker is premixed with loading buffer. The ladders are not intended for use in quantitative analysis.

 

Ready-to-use : 10 mM Tris-HCl, pH8.0, 1 mM EDTA, 5% Glycerol, 0.005% Bromophenol Blue, and 0.005% Xylene Cyanol

 

-20¡É for 2 years
4¡É for 6 months
Room temperature (20-25¡É) for 2 months

 

Concentration : 200 ng/5 ml (40 mg/ml)
Recommended loading : 5-10 ml (100-200 lanes, ready-to-use)
Range : 500 – 10,000 bp
Number of bands : 9

5 ml/200 ng/lane ; 0.7% agarose in 0.5x TAE, stained with ethidium bromide