Stepview¢â 10kD Prestained Protein Marker (Dual color)
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Detail View
20010
for research use only
• Dual color size standard (blue and pink) • 10 Kda size increment (easy size estimation) • Highly purified and blended 11 recombinant proteins • His-tag sequence in the proteins enables direct detection on western blots using anti-his antibody • User oriented ready to use solution
Mbiotech's Step-view¢â 10Kd prestained protein molecular weight standard is a dual color prestained size marker. It is a mixture of 11 recombinant purified and prestained polypeptides whose molecular weights are well-adjusted. Step-viewTM 10Kd prestained protein molecular weight standard is available in a ready-to-use formula (no need to boil before use), and is suitable for sizing proteins between 15,000 and 120,000 Da by SDS-PAGE. A blue and pink chromophore is covalently bound to recombinant proteins, and 11 prestained proteins are readily visible during electrophoresis or electrophoretic transfer from the gel to a membrane. The 50 and 100 Kda proteins labeled with pink chromophore enables easy discrimination. Because coupling of chromophore to the proteins affects their apparent molecular weights in SDS-PAGE relative to unstained proteins, unstained protein standard (Cat. No. 20011) should be used for accuracy >95%.
• Recommended loading volume: 5-10ul/lane • Typical number of lanes: 50 • Number of Bands: 11 • Range (Kd): 15-120 • Recommended storage condition: -20¡É for one year
12% Hi-Sieve¢â PAGE gel 5ul loading |
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