LandMark¢â Broad Range Prestained Protein Marker(all blue)
Cat No. Manual Product Size Storage Temp. Price(US$) Qty chk
20051 LandMark¢â Broad Range Prestained Protein Marker(all blue) 500 ul -20¡É $80
  
Detail View
  20051
• Blue dyed proteins with two contrasting bands
• Broad range size estimation(7-240kDa)
• Easy visualization during electrophoresis and evaluation of western transfer efficiency
• User oriented ready to use solution

Description

LandMarkTM broad range prestained protein marker(all blue) contains 10 blue colored polypeptides whose molecular weights are well-adjusted ranging from 7,000 to 240,000 Da. It is provided preblended in a ready-to-use formula and no reconstitution or further dilution is necessary before use. A blue chromophore is covalently bound to proteins, and 10 prestained proteins are visible during electrophoresis or electrophoretic transfer from the gel to a membrane. The 25 KDa and 70 KDa proteins serve as a landmark because they are present at a higher concentration in the mixture relative to the adjacent bands. Because coupling of chromophore to the proteins affects their apparent molecular weights in SDS-PAGE, unstained protein standard should be used for accuracy >95%.

Usage Information


• 1.5 ug/ul in 62.5 mM Tris-HCl (pH6.8), 15% Glycerol, 1% SDS, 1% b-mercaptoethanol
• Recommended loading volume: 5ul/lane
• Typical number of lanes: 100
• Number of Bands: 10
• Range (Kd): 7-240
• Recommended storage condition: -20¡É for one year
5ul/lane
7~12% SDS-PAGE