LandMark¢â Broad Range Prestained Protein Marker(dual color)
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Detail View
20050
• Dual color size standard (blue and orange) • Broad range size estimation(7-240kDa) • Highly purified and blended 10 recombinant proteins • User oriented ready to use solution
LandMark¢â broad range prestained protein marker(dual color) is a dual color prestained size marker. It is a mixture of 10 recombinant purified and prestained polypeptides whose molecular weights are well-adjusted ranging from 7,000 to 240,000 Da. It is provided preblended in a ready-to-use formula and no reconstitution or further dilution is necessary before use. A blue and pink chromophore is covalently bound to proteins, and 10 prestained proteins are visible during electrophoresis or electrophoretic transfer from the gel to a membrane. The 25 KDa and 70 KDa proteins labeled with orange chromophore offer easy identification and serve as a landmark. Because coupling of chromophore to the proteins affects their apparent molecular weights in SDS-PAGE, unstained protein standard should be used for accuracy >95%.
• 1.5 ug/ul in 62.5 mM Tris-HCl (pH6.8), 15% Glycerol, 1% SDS, 1% b-mercaptoethanol • Recommended loading volume: 5ul/lane • Typical number of lanes: 100 • Number of Bands: 10 • Range (Kd): 7-240 • Recommended storage condition: -20¡É for one year |
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5ul/lane 15% SDS-PAGE | |