West-view¢â II Western Size Marke is a brand-new, innovative true protein size marker. You can quickly and easily visualize discrete bands on your western blot only by a simple application of marker with your sample, You can get superior results, without further processing like adding extra reagents as described by many other suppliers.
All the highly purified recombinant proteins are designed to bind immunoglobulins with high affinity in a denatured form, thus marker proteins bind to primary or secondary antibodies in your western blotting. You can use it in chemiluminescent or chromogenic detection methods of your choice. It's compatible with most immunodetection methods, including horseradish peroxidase or alkaline phosphatase systems.

You'll get sharp, clear bands and precise, reproducible molecular weight estimation directly on western blot as shown on the figure.
West-view¢â II western size marker can also be used for optimizing your western transfer condition, verification of antibody conjugated peroxidase intactness, and controlling the detection system. In case, size dependent uneven band¡¯s densities of western size marker in western blotting indicate the inappropriate transfer of your proteins to membrane, and further requires adjusting protein transfer condition. No marker bands in western blot may indicate functional problems in your detection system.

• Direct marker visualization on western blots
• Easy-to-use procedure
• Accurate protein molecular weight estimation

 

• Recommended loading volume : 1-5ul/lane
• Typical number of lanes: 50-200
• Number of Bands : 7 for rabbit antibody, 8 for mouse antibody
• Range (Kd) : 16-215
• Recommended storage condition : -20¡É for one year